A microfluidic circuit on a disk platform, also known as lab-on-a-disk, is an integrated system for automated high-throughput screening for biochemical analysis. The microfluidic circuit on a disk performs biochemical analysis through sequential processes such as filtration, separation, detection, and synthesis of reagents. Sequential processes in microfluidic circuits operate through the systematically linked components, which include channels, valves, and chambers. The microchannels should have micrometer scale for precise micro-volume liquid control in the microfluidic circuit on a disk. A new concept of a 3D slope valve has been developed, which performs precise and sequential micro-volume liquid control through centrifugal and gravitational forces. Micro-volumes of liquids in a slope valve-equipped circuit are controlled over a wide range of angular velocities through the control of the valve geometry, types of liquid and volume.

As the manufacturing technology of microfluidic systems has improved, the FLISA is implemented onto the microfluidic disk platform which shows detection of trace bio-chemicals with high resolution. We suggest a novel microfluidic disk including a 3D incubation chamber using multi-material to low cost which is fabricated to simple mechanical assembly. VEGF detection is performed sequentially one-step using a 3D microfluidic disk within an hour. On the 3D microfluidic disk, the microbead-based FLISA protocol is operated from the incubation process to the detection process in hand-free without additional components to liquid control. It is possible to reduce time from the reagent loading to the detection step within an hour, in addition to benefits of reducing the amount of reagents to 1/10. For the incubation process, the microbead movement is controlled through the centrifugal force by disk rotation and gravitational force by bead sedimentation on a slope of chamber.

A micro-reaction system was designed to enable reactions involving multiple chemical reagents, thereby offering continuous operation, enhancing heat and mass transfer, reducing the reaction time and volumes, and protecting the reaction from air and moisture in a microscale device with a closed reaction environment. Microfluidic mixing increased the rate of diffusion between two reagent delivery solutions. The benefits derived from rapid diffusion have motivated progress toward device miniaturization and mass production through the integration of microscale devices into a large plant system. A continuous and efficient synthetic process was developed based on a microfluidic reactor in which was implemented a time pulsed mixing method that had been optimized using numerical simulations and experimental methods.